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Title: A STUDY ON THE EFFECT OF BED-TIME BRUSHING ON THE ORAL BACTERIAL COUNTS USING QUANTITATIVE PCR METHOD
A study on the effect of bedtime brushing on oral bacterial counts using the quantitative PCR method.
Everybody knows brushing their teeth is good for oral hygiene. However, not everyone is aware that during night sleep when saliva does not do flushing action, bacteria grow a lot and cause heavy damage not only to teeth (periodontitis or gum disease) but also play a role in causing some deadly diseases like heart disease, cancer, diabetes, etc.
Midnight snackers and those who eat sweets and starchy food at dinner, do not know how much bacteria grows in their mouths if they ignore brushing their teeth.
My hypothesis is that about a 50% decrease in the bacterial count will be observed upon bedtime brushing.
10 volunteers were asked to eat candy at night and go to bed with and without brushing their teeth.
A quantitative Polymerase Chain Reaction (qPCR) analysis was done to measure the bacterial count in the morning saliva after eating a sweet in the previous night and going to bed with and without brushing teeth.
The results showed that upon brushing on average there was a 61% decrease in bacterial growth in 90% of the volunteers. Though many studies show brushing decreases oral bacterial count, this is a unique study that does an analysis of morning saliva following bedtime brushing.
The objective of this study is to give awareness about the importance of bedtime brushing quality on oral hygiene to prevent many deadly diseases and enjoy good health.
Reference: Steven Jaksha, "The Role of a Toothbrush in Tooth Brushing, Intra-oral Bacteria." AAOSH. N.p., n.d. April 20, 2011.
Awareness Given Through This Research
Dental Awareness Camp 2014 - at a Middle School in a remote village in South India. This camp brought awareness of the importance of oral cleaning & bedtime brushing and motivated them to maintain good oral hygiene.