A study on the effect of bed-time brushing on the oral bacterial counts using quantitative PCR method
- Everybody knows brushing the teeth is good for oral hygiene. However not everyone is aware that during night sleep when saliva does not do flushing action, bacteria grows a lot and causes
heavy damage not only to teeth (periodontitis or gum disease) but also play a role in causing some deadly diseases like heart disease, cancer, diabetes, etc.
- Mid-night snackers and those who eat sweets and starchy food at dinner, do not know how much bacteria grows in their mouth, if they ignore brushing their teeth.
- My hypothesis is that about 50% decrease in bacterial count will be observed upon bedtime brushing.
- 10 volunteers were asked to eat a candy at night and go to bed with and without brushing the teeth.
- A quantitative Polymerase Chain Reaction (qPCR) analysis was done to measure the bacterial count in the morning saliva after eating a sweet in the previous night and going to bed with and
without brushing teeth.
- The results showed that upon brushing on an average there was 61% decrease in bacterial growth in 90% of the volunteers. Though many studies show brushing decreases oral bacterial count,
this is a unique study which does analysis in the morning saliva following bed-time brushing.
- The objective of this study is to give awareness about the importance of bed time brushing quality on the oral hygiene to prevent many deadly diseases and enjoy good
Reference: Steven Jaksha, "The Role of a Toothbrush in Tooth Brushing, Intra-oral Bacteria." AAOSH. N.p., n.d. April 20, 2011.